Separation and purification method for high-purity monomer sweroside series components

The invention provides a separation and purification method for high-purity monomer sweroside series components. The method comprises the following steps: S1) extracting a raw material with alcohol, thereby acquiring an extracting solution, wherein a swertia plant or medicinal material is taken as the raw material; S2) performing anti-phase polymer chromatography packing adsorption on the extracting solution, and then eluting, thereby acquiring an eluant; S3) performing silica column chromatography purification on the eluant, thereby acquiring a purified eluant; and S4) performing the preparing type high performance liquid chromatography separation on the purified eluant, thereby acquiring high-purity monomer sweroside series components. Compared with the prior art, the separation and purification method provided by the invention has the advantages that the process is simple, the cost is low, various different sweroside series components can be acquired, and the product yield and purity are both higher. 本发明提供