System and methods of detecting and demonstrating ultraviolet damage to hair via evaluation of protein fragments

The present invention is directed to a method to measure ultraviolet or copper damage of hair comprising: eluting a protein fragment from a hair sample with an aqueous solution; extracting the proteins using a solvent; analyzing the protein fragment samples with MALDI-MS; resulting in protein fragment results; identifying presence of a marker protein fragment and identifying what the fragment is by indentifying the amino acid sequence using high resolution Orbitrap -MS wherein the protein fragment is a protein fragment of S100A3. 本发明涉及用于测量毛发的紫外线损伤或铜损伤的方法，所述方法包括：用水性溶液从毛发样品洗脱蛋白片段；使用溶剂提取蛋白；采用MALDI-MS对蛋白片段样品进行分析；得到蛋白片段结果；鉴定标记蛋白片段的存在，并通过用高分辨率Orbitrap-MS鉴定氨基酸序列来鉴别该片段为哪种，其中蛋白片段是S100A3的蛋白片段。