Isothermal amplication detection kit for nucleic acid of aeromonas and detection method of isothermal amplication detection kit
The invention provides an isothermal amplication detection kit for a nucleic acid of aeromonas. The isothermal amplication detection kit comprises a grinding liquid tube, a nucleic acid extract solution A tube, a nucleic acid extract solution B tube, a nucleic acid extract solution C tube, a TE buff...
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| creator | BU JIALI HE LIN |
| description | The invention provides an isothermal amplication detection kit for a nucleic acid of aeromonas. The isothermal amplication detection kit comprises a grinding liquid tube, a nucleic acid extract solution A tube, a nucleic acid extract solution B tube, a nucleic acid extract solution C tube, a TE buffer liquid tube, a UNG enzyme tube, an LAMP reaction liquid tube, a Bst DNA polymerase tube, a color developing agent tube, a positive control nucleic acid tube and a negative control tube. Common aeromonas bacterial can be effectively detected, and the on-site, programmed and standardized detection process is achieved. The invention further provides a detection method for the aeromonas. A target gene segment is optimized to design a LAMP specific primer, so that the aeromonas bacterial can be effectively detected by the primer, the detection specificity is good and the detection result is very easy to distinguish.
本发明提供了种气单胞菌核酸等温扩增检测试剂盒，包括研磨液管、核酸提取液A管、核酸提取液B管、核酸提取液C管、TE缓冲液管、UNG酶管、LAMP反应液管、Bst DNA聚合酶管、显色剂管、阳性对照核酸管和阴 |
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本发明提供了种气单胞菌核酸等温扩增检测试剂盒，包括研磨液管、核酸提取液A管、核酸提取液B管、核酸提取液C管、TE缓冲液管、UNG酶管、LAMP反应液管、Bst DNA聚合酶管、显色剂管、阳性对照核酸管和阴</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROCESSES USING MICROORGANISMS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2017</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170510&DB=EPODOC&CC=CN&NR=106636394A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170510&DB=EPODOC&CC=CN&NR=106636394A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>BU JIALI</creatorcontrib><creatorcontrib>HE LIN</creatorcontrib><title>Isothermal amplication detection kit for nucleic acid of aeromonas and detection method of isothermal amplication detection kit</title><description>The invention provides an isothermal amplication detection kit for a nucleic acid of aeromonas. The isothermal amplication detection kit comprises a grinding liquid tube, a nucleic acid extract solution A tube, a nucleic acid extract solution B tube, a nucleic acid extract solution C tube, a TE buffer liquid tube, a UNG enzyme tube, an LAMP reaction liquid tube, a Bst DNA polymerase tube, a color developing agent tube, a positive control nucleic acid tube and a negative control tube. Common aeromonas bacterial can be effectively detected, and the on-site, programmed and standardized detection process is achieved. The invention further provides a detection method for the aeromonas. A target gene segment is optimized to design a LAMP specific primer, so that the aeromonas bacterial can be effectively detected by the primer, the detection specificity is good and the detection result is very easy to distinguish.
本发明提供了种气单胞菌核酸等温扩增检测试剂盒，包括研磨液管、核酸提取液A管、核酸提取液B管、核酸提取液C管、TE缓冲液管、UNG酶管、LAMP反应液管、Bst DNA聚合酶管、显色剂管、阳性对照核酸管和阴</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROCESSES USING MICROORGANISMS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2017</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNzDEKAjEQheE0FqLeYTyAoEQClrIo2ljZL0MyYQeTTNiMtVcXFgvLrd5ffLyl-dyb6EBjxgSYa2KPylIgkJKf6sUKUUYob5-IPaDnABIBaZQsBRtgCX8-kw4yCZ5xvTaLiKnR5rcrs71ent1tR1V6ahU9FdK-exz2zllnT8eznWO-Rw1Jjw</recordid><startdate>20170510</startdate><enddate>20170510</enddate><creator>BU JIALI</creator><creator>HE LIN</creator><scope>EVB</scope></search><sort><creationdate>20170510</creationdate><title>Isothermal amplication detection kit for nucleic acid of aeromonas and detection method of isothermal amplication detection kit</title><author>BU JIALI ; HE LIN</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN106636394A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2017</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROCESSES USING MICROORGANISMS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>BU JIALI</creatorcontrib><creatorcontrib>HE LIN</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>BU JIALI</au><au>HE LIN</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Isothermal amplication detection kit for nucleic acid of aeromonas and detection method of isothermal amplication detection kit</title><date>2017-05-10</date><risdate>2017</risdate><abstract>The invention provides an isothermal amplication detection kit for a nucleic acid of aeromonas. The isothermal amplication detection kit comprises a grinding liquid tube, a nucleic acid extract solution A tube, a nucleic acid extract solution B tube, a nucleic acid extract solution C tube, a TE buffer liquid tube, a UNG enzyme tube, an LAMP reaction liquid tube, a Bst DNA polymerase tube, a color developing agent tube, a positive control nucleic acid tube and a negative control tube. Common aeromonas bacterial can be effectively detected, and the on-site, programmed and standardized detection process is achieved. The invention further provides a detection method for the aeromonas. A target gene segment is optimized to design a LAMP specific primer, so that the aeromonas bacterial can be effectively detected by the primer, the detection specificity is good and the detection result is very easy to distinguish.
本发明提供了种气单胞菌核酸等温扩增检测试剂盒，包括研磨液管、核酸提取液A管、核酸提取液B管、核酸提取液C管、TE缓冲液管、UNG酶管、LAMP反应液管、Bst DNA聚合酶管、显色剂管、阳性对照核酸管和阴</abstract><oa>free_for_read</oa></addata></record> |
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| language | chi ; eng |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Isothermal amplication detection kit for nucleic acid of aeromonas and detection method of isothermal amplication detection kit |
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