Isothermal amplication detection kit for nucleic acid of aeromonas and detection method of isothermal amplication detection kit

The invention provides an isothermal amplication detection kit for a nucleic acid of aeromonas. The isothermal amplication detection kit comprises a grinding liquid tube, a nucleic acid extract solution A tube, a nucleic acid extract solution B tube, a nucleic acid extract solution C tube, a TE buffer liquid tube, a UNG enzyme tube, an LAMP reaction liquid tube, a Bst DNA polymerase tube, a color developing agent tube, a positive control nucleic acid tube and a negative control tube. Common aeromonas bacterial can be effectively detected, and the on-site, programmed and standardized detection process is achieved. The invention further provides a detection method for the aeromonas. A target gene segment is optimized to design a LAMP specific primer, so that the aeromonas bacterial can be effectively detected by the primer, the detection specificity is good and the detection result is very easy to distinguish. 本发明提供了种气单胞菌核酸等温扩增检测试剂盒，包括研磨液管、核酸提取液A管、核酸提取液B管、核酸提取液C管、TE缓冲液管、UNG酶管、LAMP反应液管、Bst DNA聚合酶管、显色剂管、阳性对照核酸管和阴