Molecular specific labeling primer of physalis minima and detection method

The invention relates to a molecular specific labeling primer of physalis minima and a method for rapidly detecting the physalis minima by utilizing the molecular specific labeling primer. Primer sequences are as follows: an upstream primer XSJF: 5'-TTGCAAACTATCTTGTGAGTCG-3'; a downstream primer XSJR: 5'-CCACCAGAATGTACTTGTTACG-3'. By applying the specific primer XSJF/XSJR designed by the invention, rapid and accurate identification of the physalis minima can be realized through a PCR (Polymerase Chain Reaction) method. The XSJF/XSJR is a specific amplification primer of the physalis minima; if the primer is used for detecting other physalis plant samples, the primer has a negative reaction; the method is simple and convenient, high in sensitivity, accurate in result and short in consumed time. 本发明涉及小酸浆的分子特异性标记引物，以及种利用该特异性引物对小酸浆进行快速检测的方法。所述引物序列为：上游引物XSJF:5'-TTGCAAACTATCTTGTGAGTCG-3'；下游引物XSJR:5'-CCACCAGAATGTACTTGTTACG-3'。运用本发明设计的特异性引物XSJF/XSJR，通过PCR方法，可以实现小酸浆的快速准确鉴别。XSJF/XSJR是小酸浆专性扩增引物，若为其他酸浆属植物样品，则为阴性反应；本发明方法简