Paeonia rockii primary culture callus induction method

The invention discloses a paeonia rockii primary culture callus induction method. The paeonia rockii primary culture callus induction method comprises the following steps: (1) explant selection: selecting bulbel of paeonia rockii as an explant, washing, and pre-cooling for 24 to 48 hours; (2) sterilizing and inoculating: stripping bud scales off from the bulbel obtained in step (1) under an aseptic condition, sterilizing, and then inoculating the bulbel to a callus induction culture medium; (3) callus induction culture: putting the inoculated explant in a culture room for culturing, and culturing for 7 to 10 days by using red light. Compared with the prior art, the paeonia rockii primary culture callus induction method disclosed by the invention has the advantages that an explant pre-cooling method is adopted, specific culture medium components are combined, a red-light culture method is finally adopted, the browning rate of the paeonia rockii can be effectively reduced to be 0, no browning phenomenon happens