Method of using spermine to induce tobacco embryoid

The invention discloses a method of using spermine to induce tobacco embryoid. The method includes: using spermine as an inducer to prepare an inducing culture medium for embryoid induction of tobacco leaf explant. Compared with conventional culture media, the inducing culture medium prepared by taking spermine as the inducer is high in tobacco embryoid forming speed, yield and quality, and the objective of producing tobacco embryoid can be achieved directly without transferring original explant into other bottles for subculture. 本发明公开了种用精胺诱导烟草胚状体的方法，以精胺作诱导剂制备诱导培养基进行烟草叶片外植体的胚状体诱导。本发明以精胺作诱导剂制备的诱导培养基比传统的培养基诱导烟草胚状体形成速度快、产量高、质量好，且原始外植体无需转瓶进行继代培养，即可直接达到生产烟草胚状体的目的。