Method for inducing regenerated plant of tobacco leaf by utilizing Lingfasu

The invention discloses a method for inducing a regenerated plant of tobacco leaf by utilizing Lingfasu. The method comprises the following steps: taking tobacco leaf explants; inoculating to a MS solid culture medium added with Lingfasu; regulating pH to 5.8-6.0; and culturing for 30 days, thereby acquiring the regenerated plant of tobacco. According to the invention, the preparation for the culture medium is simplified. The method has the advantages of simple operation, high speed in inducing the regenerated plant, high yield and high quality. 本发明公开了种用灵发素诱导烟草叶片再生植株的方法，取烟草叶片外植体，接种至添加有灵发素的MS固体培养基中，调节pH至5.8-6.0，培养30天，获得烟草再生植株。本发明能够简化了培养基的配制，具有操作简单诱导再生植株速度快、产量高、质量好的优点。