Preparation method of jettisonable aptamer sensor for OTA sensitivity detection

The invention provides a preparation method of a jettisonable aptamer sensor for OTA (ochratoxin) sensitivity detection. The method includes the steps of: S1. preparation of an MPA modified nano colloidal gold solution; S2. activation of an aptamer; S3. preparation of a PDDA modified screen-printed electrode; S4. preparation of a gold nanoparticle modified screen-printed electrode; and S5. preparation of aptamer modified Au NPs-SPCE. Compared with the traditional detection method, the label-free impedance detection method of OTA proposed in the invention has the advantages of simpler and more flexible operation, simpler instrument and equipment, small reagent dosage, and low detection cost, etc. 本发明提供了种用于OTA灵敏检测的可抛式适配体传感器的制备方法，步骤如下：步骤1、MPA修饰的纳米胶体金溶液的制备；步骤2、适配体的活化；步骤3、PDDA修饰的丝网印刷电极的制备；步骤4、金纳米粒子修饰的丝网印刷电极的制备；步骤5、适配体修饰的Au NPs-SPCE的制备。与传统检测方法相比，本发明中所提出的OTA的免标记阻抗检测方法具有操作更简便灵活，仪器设备更简单，试剂用量少，检测成本低廉等特点。