Preservation method of human amniotic mesenchymal stem cells
The invention relates to a preservation method of human amniotic mesenchymal stem cells, and belongs to the biotechnology field. A human amniotic mesenchymal stem cell preservation solution is prepared from 12-15 g of sodium cirate (C6H5Na3O7.2H2O), 3-6 g of citric acid (C6H8O7.H2O), 12-16 g of gluc...
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Zusammenfassung: | The invention relates to a preservation method of human amniotic mesenchymal stem cells, and belongs to the biotechnology field. A human amniotic mesenchymal stem cell preservation solution is prepared from 12-15 g of sodium cirate (C6H5Na3O7.2H2O), 3-6 g of citric acid (C6H8O7.H2O), 12-16 g of glucose (C6H12O6.H2O), 10 ml of human plasma, 10 g of human albumin and 990 ml of water. The preservation method has the advantages that the human amniotic mesenchymal stem cell preservation solution does not contain animal serum and has the high safety; experiments prove that the human amniotic mesenchymal stem cells still can express following three kinds of mesenchymal stem cell membrane molecules including a human leukocyte differentiation antigen CD73, a human leukocyte differentiation antigen CD90 and a human leukocyte differentiation antigen CD105 after being preserved in the preservation solution for 24 hours at 4 DEG C.
本发明涉及种人羊膜间充质干细胞的保存方法,属于生物技术领域。人羊膜间充质干细胞保存液,由下述成分组成:枸橼酸钠(CHNaO·2HO)12-15g,枸橼酸(CHO·HO)3-6g,葡萄 |
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