High purity low endotoxin carbohydrate (HPLE) compositions, and methods of isolation thereof

Provided herein is a highly pure carbohydrate composition, and a method of making a highly pure carbohydrate composition. The method includes passing an aqueous carbohydrate solution through an anion exchange chromatography column including a polyethyleneimine (PEI) chromatographic media to obtain a purified solution, and isolating a highly pure carbohydrate composition from the purified solution. 本发明提供种高纯度碳水化合物组合物，及种制备高纯度碳水化合物组合物的方法。该方法包括使碳水化合物水溶液通过包含聚乙烯亚胺(PEI)色谱介质的阴离子交换色谱柱以获得经纯化的溶液，及从该经纯化的溶液中分离高纯度碳水化合物组合物。