Cylas formicarius DNA fast extraction method and application thereof

The invention discloses a cylas formicarius DNA (Deoxyribonucleic Acid) fast extraction method and application thereof. According to the method, cylas formicarius imagoes are subjected to elytron removal and are cleaned by sterilized ddH2O; after the drying on sterilized filter paper, single-head cy...

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Hauptverfasser: YANG ZIXIANG, WEI YUNGUI, SUN YUEXIAN, HUANG QIONG, XU KECHENG, ZHANG YU, DONG YUELI, ZHANG ZHICHAO, SONG HONG, LI YUNFEI
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a cylas formicarius DNA (Deoxyribonucleic Acid) fast extraction method and application thereof. According to the method, cylas formicarius imagoes are subjected to elytron removal and are cleaned by sterilized ddH2O; after the drying on sterilized filter paper, single-head cylas formicarius is taken and is put into a 1.5ml centrifugal tube; the sufficient grinding is performed by a sterilized glass rod; 150 [mu]l of suspended 5-percent chelex-100 solution is added; metal bath is performed for 5min at 100 DEG C; centrifugation being 12000 r/min is performed for 5 min; and obtained supernate is DNA extraction liquid. 1[mu]l of the DNA extraction liquid is taken, and can be directly used as a DNA template for PCR (Polymerase Chain Reaction) amplification reaction. The whole extraction process is completed about 15 minutes; the cost of each sample is 1.5 Yuan; and the method is the fastest and cheapest cylas formicarius DNA efficient extraction method at present. The extraction method has