Directional differentiation system and method for liver stem cells
The invention relates to the technical field of biomedical engineering, particularly to a directional differentiation system and method for liver stem cells for long term invitro culture of liver stem cells. The directional differentiation system comprises an expansion culture medium with explicit c...
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| Format: | Patent |
| Sprache: | chi ; eng |
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| Zusammenfassung: | The invention relates to the technical field of biomedical engineering, particularly to a directional differentiation system and method for liver stem cells for long term invitro culture of liver stem cells. The directional differentiation system comprises an expansion culture medium with explicit chemical compositions, which is used for invitro culture of mouse or human liver stem cells, and a differentiation culture medium with explicit chemical compositions, which is used for changing the mouse or human liver stem cells to be mature liver cells through induced differentiation. According to the system and method provided by the invention, liver stem cells from mouse fetal liver tissue or hepatic progenitor cells obtained through human pluripotent stem cell differentiation are selectively expanded, the liver stem cells are cultivated for more than 20 generations under the condition, and stable liver stem cell molecular phenotype can be maintained; further, the cultured mouse or human liver stem cells are cha |
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