Bioluminescence detection kit of human carboxylesterase 1 as well as use method and application of bioluminescence detection kit

The invention relates to a bioluminescence detection kit of human carboxylesterase 1 as well as a use method and application of the bioluminescence detection kit. The kit comprises a liquid A, a liquid B, a liquid C and a quality control standard substance, wherein the liquid A is 100mM of phosphate buffer liquid with pH of 5.5-10, preferably, the buffer system with pH of 6.5; the liquid B is 0.1-10mM of specificity bioprobe substrate liquid containing the human carboxylesterase 1, and a solvent is dimethyl sulfoxide; the liquid C is a fluorescein detection reagent; the quality control standard substance is 5mg/ml of the phosphate liquid of hCE1 with pH of 6.5. The specificity bioprobe substrate containing the human carboxylesterase 1 is 4,5-dihydrogen-2(6-hydroxy-2-phenyl imidazole)4-carboxylic acid triazote methyl ester or 4,5-dihydrogen-2(6-amino-2-phenyl imidazole)4-carboxylic acid triazote methyl ester; the kit can be used for detecting the presence or absence of hCE1 in different biological samples and