Method and system for suspension culture

The present invention relates to cell culture in bioreactors, such as flexible cellbag bioreactors. More closely the invention relates to a method and system for determining the cell density in a bioreactor culture and for controlling the perfusion rate of a suspension culture of cells in a bioreactor, comprising measuring the oxygen uptake of primary mononuclear cells in a non-static bioreactor. 本发明涉及诸如柔性细胞袋生物反应器之类的生物反应器中的细胞培养。更详尽地，本发明涉及用于确定生物反应器培养物中的细胞密度且用于控制生物反应器中的细胞的悬浮培养物的灌注速率的方法和系统，所述方法和系统包括测量非静止的生物反应器中的原代单核细胞的氧摄取量。