Florescent real-time quantitative PCR method for rapidly detecting first component content of dermatophagoides farinae allergen in house dust

The method discloses a florescent real-time quantitative PCR method for rapidly detecting a first component content of dermatophagoides farinae allergen in house dust. According to the method, Der f 1 recombinant plasma is taken as a standard substance, a florescent real-time quantitative PCR and a TaqMan probe of the Der f 1 are designed; and by virtue of the TaqMan probe florescent real-time quantitative PCR method, the rapid quantitative detection of the Der f 1 content of the dermatophagoides farinae allergen in the house dust is achieved by constructing a Der f 1 florescent real-time quantitative PCR standard curve. The method provided by the invention detects a same house dust sample respectively by virtue of the environment-friendly and cheap Der f 1 florescent real-time quantitative PCR method and a commercial and expensive ELISA kit, and a correlation coefficient R between the two methods is equal to 0.94, wherein P is less than 0.05, showing that the two methods keep a good linear relation. 本发明公开了种快