Shellfish vibrio parahemolyticus LAMP detection kit and application thereof

The invention discloses shellfish vibrio parahemolyticus detection primer which is prepared from outside upstream primer F3, outside downstream primer B3, inside upstream primer FIP, inside downstream primer BIP, annular upstream primer LF and annular downstream primer LB. The invention further disc...

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Bibliographische Detailangaben
Hauptverfasser:	HAN FENGJIE, WANG XUEPENG, FU TIANZENG, WU JIAYAN, LI YU, PANG XIAORU, LI QIMENG
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention discloses shellfish vibrio parahemolyticus detection primer which is prepared from outside upstream primer F3, outside downstream primer B3, inside upstream primer FIP, inside downstream primer BIP, annular upstream primer LF and annular downstream primer LB. The invention further discloses a shellfish vibrio parahemolyticus LAMP detection kit and application thereof. A detection method of the shellfish vibrio parahemolyticus LAMP detection kit can quickly, conveniently and accurately detect pathogene; furthermore, the detection method is high in sensitivity; the results show that the LAMP detection method is strong in specificity by amplifying 10 strains of vibrio parahemolyticus and 20 strains of other vibrio; compared with a general PCR detection method, the detection method is high in sensitivity, and the sensitivity can reach 88fg/LAMP; in addition, whether byproducts are generated can be directly observed through eyes according to the LAMP detection method, and the reaction results can be