High-throughput screening method for obtaining apramycin high-yield strain

The invention relates to a high-throughput screening method for obtaining an apramycin high-yield strain. The high-throughput screening method comprises the following steps: (1) taking streptomyces tenebrarius spores into sterile water, and uniformly mixing to prepare a streptomyces actuosus spore suspension; (2) transferring the streptomyces tenebrarius spore suspension into a sterilized culture dish, blow-drying through sterile wind to prepare a pellicle, then carrying out injection induction of a low-energy ion beam, then carrying out ultraviolet radiation, washing by virtue of the sterile water, and then coating a plate culture medium to prepare a single colony; (3) inoculating the single colony in a pore plate culture medium within a pore plate, and carrying out shake culture to prepare a culture solution; and (4) transferring the culture solution into a bioassay culture medium plate containing bacillus subtilis and carrying out inhibition detection, selecting single bacteria with inhibition zone diamete