Clarias gariepinus antimicrobial peptide and extraction method thereof
The invention belongs to the field of biological purification, particularly provides clarias gariepinus antimicrobial peptide and further provides a purification and extraction method of the antimicrobial peptide. The method specifically comprises the steps that (1) raw materials are subjected to pr...
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| creator | FAN TIANGUO GAO JINWEI WANG XIAOMEI LI TAO CHEN CHENGXUN |
| description | The invention belongs to the field of biological purification, particularly provides clarias gariepinus antimicrobial peptide and further provides a purification and extraction method of the antimicrobial peptide. The method specifically comprises the steps that (1) raw materials are subjected to primary processing, specifically, a clarias gariepinus processing by-product is cut into pieces under an ice bath, and the pieces are grinded through a texture homogenizer and boiled in a sealed container; (2) enzymolysis is carried out, specifically, a buffer solution is added to the raw materials in the step (1) to be mixed, and then proteases are added for enzymolysis; (3) separation is carried out, specifically, enzymatic hydrolysate of the step (2) is subjected to enzyme inactivation firstly, then supernatant is collected through centrifugation, a certain amount of saccharose is added, then the mixture is frozen and dried, and a coarse antimicrobial peptide product is obtained; and (4) purification is carried ou |
| format | Patent |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE |
| title | Clarias gariepinus antimicrobial peptide and extraction method thereof |
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