Clarias gariepinus antimicrobial peptide and extraction method thereof

The invention belongs to the field of biological purification, particularly provides clarias gariepinus antimicrobial peptide and further provides a purification and extraction method of the antimicrobial peptide. The method specifically comprises the steps that (1) raw materials are subjected to pr...

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Bibliographische Detailangaben
Hauptverfasser: FAN TIANGUO, GAO JINWEI, WANG XIAOMEI, LI TAO, CHEN CHENGXUN
Format: Patent
Sprache:chi ; eng
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Beschreibung
Zusammenfassung:The invention belongs to the field of biological purification, particularly provides clarias gariepinus antimicrobial peptide and further provides a purification and extraction method of the antimicrobial peptide. The method specifically comprises the steps that (1) raw materials are subjected to primary processing, specifically, a clarias gariepinus processing by-product is cut into pieces under an ice bath, and the pieces are grinded through a texture homogenizer and boiled in a sealed container; (2) enzymolysis is carried out, specifically, a buffer solution is added to the raw materials in the step (1) to be mixed, and then proteases are added for enzymolysis; (3) separation is carried out, specifically, enzymatic hydrolysate of the step (2) is subjected to enzyme inactivation firstly, then supernatant is collected through centrifugation, a certain amount of saccharose is added, then the mixture is frozen and dried, and a coarse antimicrobial peptide product is obtained; and (4) purification is carried ou