Novel composite protective agent for freeze-dried preparation of staphylococcus aureus

The invention provides a novel composite protective agent for freeze-dried preparation of staphylococcus aureus. The protective agent mainly contains the ingredients by content: 2% to 6% of trehalose, 12% to 18% of monosodium glutamate, 0.5% to 1.5% of L-cysteine hydrochloride and 0.25% to 0.75% of...

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Bibliographische Detailangaben
Hauptverfasser:	LIU YI, HU HAIRONG, LI ZAN, LIU BO, YANG HAILI, LI JINXIA, CHENG CHI, DU HAIBO
Format:	Patent
Sprache:	chi ; eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a novel composite protective agent for freeze-dried preparation of staphylococcus aureus. The protective agent mainly contains the ingredients by content: 2% to 6% of trehalose, 12% to 18% of monosodium glutamate, 0.5% to 1.5% of L-cysteine hydrochloride and 0.25% to 0.75% of bovine serum albumin. The freeze-drying protective agent is mainly applied to the preparation of freeze-dried bacterium powder of the staphylococcus aureus. The bacterium powder of the staphylococcus aureus is obtained through subjecting staphylococcus aureus thalli to culture, centrifugation and washing, then, carrying out collecting, carrying out mixing with the protective agent before carrying out freeze-drying, carrying out subpackaging, carrying out pre-freezing for 2 to 3 hours at the temperature of -80 DEG C, and then, carrying out freeze drying for 16 to 18 hours. Through using the protective agent during the preparation of the bacterium powder, the freezing resistance of the thalli can be improved, so that