Method for extracting and purifying tacrolimus from fermentation broth

The invention provides a method for extracting and purifying tacrolimus from a fermentation broth. The method comprises: carrying out solid-liquid separation on a fermentation broth, and collecting the filtration residue; extracting with acetone to obtain an extracting liquid; concentrating the extracting liquid, treating with a macroporous resin, and collecting a desorption liquid; treating the desorption liquid to obtain a liquid to be chromatographed; carrying out silica gel column chromatography, and collecting a chromatography liquid; and treating the chromatography liquid, carrying out pressure reducing concentration to obtain a third concentrated liquid, adding ethyl acetate, extracting, adding n-hexane, cooling to a temperature of 1-10 DEG C, crystallizing, collecting the crystal, and carrying out vacuum drying to obtain tacrolimus. According to the present invention, the invention belongs to the technical field of biomedicine, wherein the purity of tacrolimus is improved, the content of the finally o