Method for preparing and detecting intermediate and corresponding isomer of afatinib
The invention discloses a method for simultaneously detecting a key intermediate (formula 2) and a corresponding isomer (formula 3) of afatinib. According to the method, isocratic elution is performed on a high performance liquid chromatograph; a chromatographic column which takes cellulose-tris(3,5...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention discloses a method for simultaneously detecting a key intermediate (formula 2) and a corresponding isomer (formula 3) of afatinib. According to the method, isocratic elution is performed on a high performance liquid chromatograph; a chromatographic column which takes cellulose-tris(3,5-dimethyl phenylcarbamate) as a filling agent is adopted; a flow phase is a mixed solution of n-hexane, isopropyl alcohol and acetonitrile. Through the method, the key intermediate (formula 2) and the corresponding isomer (formula 3) of the afatinib can be effectively separated and detected, and the separation degree can reach 2.0 or higher.
本发明涉及种可同时检测阿法替尼的关键中间体(式2)及其对映异构体(式3)的方法。该方法是在高效液相色谱仪上进行等度洗脱,采用纤维素-三(3,5-二甲基苯基氨基甲酸酯)为填充剂的色谱柱,流动相是正己烷、异丙醇、乙腈的混合溶液。该方法能够有效的分离和检测阿法替尼的关键中间体(式2)及其对映异构体(式3),分离度可达2.0以上。 |
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