Method for detecting methylation of wheat gamma-gliadin promoter region
The invention belongs to the technical field of biological engineering, and in particular relates to a method for detecting whether a wheat gamma-gliadin promoter region is methylated or not. The wheat gamma-gliadin is gamma-gliadin TaWG04, and a promoter is a promoter pTaWG04 used for promoting the...
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| creator | LEI ZHENSHENG HE SHENGLIAN CHAO YUE'EN WANG MEIFANG LI WENXU LIU JIAPING QIN MAOMAO ZHOU ZHENGFU WU ZHENGQING YANG PAN WANG YAHUAN WANG QINGCHANG XU FUXIN LI WEI LIU CONGCONG CHANG YANG ZHANG KUN |
| description | The invention belongs to the technical field of biological engineering, and in particular relates to a method for detecting whether a wheat gamma-gliadin promoter region is methylated or not. The wheat gamma-gliadin is gamma-gliadin TaWG04, and a promoter is a promoter pTaWG04 used for promoting the gamma-gliadin TaWG04 to perform translation and expression. When cytosine at -749bp of the promoter pTaWG04 and cytosine at -729bp of the promoter pTaWG04 are subjected to methylated modification, the expression of the gamma-gliadin TaWG04 is inhibited. The methylation detection method provided by the application is specifically used for detecting the wheat gamma-gliadin promoter region, and has the advantages of being short in detection period, high in specificity, high in accuracy, and the like; the method can be used for judging whether the promoter is methylated or not so as to effectively identify the expression status of the gamma-gliadin TaWG04 in wheat varieties, thus laying theoretical basis and applicati |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | Method for detecting methylation of wheat gamma-gliadin promoter region |
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