Method for detecting methylation of wheat gamma-gliadin promoter region

The invention belongs to the technical field of biological engineering, and in particular relates to a method for detecting whether a wheat gamma-gliadin promoter region is methylated or not. The wheat gamma-gliadin is gamma-gliadin TaWG04, and a promoter is a promoter pTaWG04 used for promoting the gamma-gliadin TaWG04 to perform translation and expression. When cytosine at -749bp of the promoter pTaWG04 and cytosine at -729bp of the promoter pTaWG04 are subjected to methylated modification, the expression of the gamma-gliadin TaWG04 is inhibited. The methylation detection method provided by the application is specifically used for detecting the wheat gamma-gliadin promoter region, and has the advantages of being short in detection period, high in specificity, high in accuracy, and the like; the method can be used for judging whether the promoter is methylated or not so as to effectively identify the expression status of the gamma-gliadin TaWG04 in wheat varieties, thus laying theoretical basis and applicati