Primer composition for detecting Claudin5 gene expression level and application of primer composition

The invention discloses a primer composition for detecting a mouse Claudin5 gene expression level and an application of the primer composition. The primer composition is composed of a primer group 1 and a primer group 2, wherein the primer group 1 is applicable to Claudin5 gene detection and the primer group 2 is applicable to Actin reference gene detection; and the sequences of the primer group 1 and the primer group 2 are shown as SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4. The invention also relates to the application of the primer composition in preparing a reagent for detecting the mouse Claudin5 gene expression level. According to the adopted technical scheme, in comparison with the prior art, the primer composition can achieve the specific detection of the Claudin5 gene expression level, so as to avoid interference of homologous genes; the primer composition is quite high in detection sensitivity, free from the use of a fluorescent probe, simple in application, accurate and efficient in dete