Method for extracting DNA of fertilized egg genome of ascaris suum

The invention provides a method for efficiently extracting DNA of a fertilized egg genome of ascaris suum. The method comprises the following five steps: washing eggs with distilled water; pre-treating the eggs; digesting protease K; removing impurities; and eluting DNA with high-purity water. The extracted DNA of the fertilized egg genome of ascaris suum has stable quality and can be used for effectively performing downstream PCR amplification, and an important technical method is provided to studying of molecular epidemiology, genetic diversity, genetic structure, systemic embryology and phylogeography of ascaris suum. The method has an important meaning to molecular diagnosis, prevention and control on porcine ascariasis. 种高效提取猪蛔虫受精卵基因组DNA的方法，包括蒸馏水洗虫卵、预处理虫卵、蛋白酶K消化、除杂质、高纯水洗脱DNA等五个步骤。本发明提取的猪蛔虫受精卵基因组DNA，质量稳定，能有效地进行下游PCR扩增，为猪蛔虫的分子流行病学、遗传多样性、遗传结构、系统发生学、谱系地理学研究提供重要的技术方法，该发明对猪蛔虫病的分子诊断和防控也有重要意义。