Culture method of magnetotactic bacteria AMB-1

The invention discloses a culture method of magnetotactic bacteria AMB-1. The culture method comprises the following steps that 1, activating bacteria; 2, obtaining first enlarged culture bacteria; 3, obtaining second enlarged culture bacteria; 4, obtaining third enlarged culture bacteria; 5, taking...

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Bibliographische Detailangaben
Hauptverfasser: LIU HUAQING, YU RUQI, LIU YUANGANG, HONG YAZHEN, WANG SHIBIN
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a culture method of magnetotactic bacteria AMB-1. The culture method comprises the following steps that 1, activating bacteria; 2, obtaining first enlarged culture bacteria; 3, obtaining second enlarged culture bacteria; 4, obtaining third enlarged culture bacteria; 5, taking a proper amount of the third enlarged culture bacteria liquid obtained in the step 4 to be inoculated into a sterile fluid nutrient medium 1653 MSMG, and conducting fermenter culture; 6, conducting centrifugation to collect the bacteria in the materials obtained in the step 5, and conducting ultrasonication washing, purification and freeze drying to obtain magnetosomes. According to the method, the aim that the AMB-1 bacteria density and the magnetosome yield are greatly improved though fermenter culture can be achieved under the conditions that only the addition amount of an iron source is changed and pH is controlled to be constant. 本发明公开了种趋磁细菌AMB-1的培养方法,包括如下步骤:(1)菌种活化;(2)第扩大培养菌;(3)第二扩大培养菌;(4)第三扩大培养菌;(5)取适量步骤(4)