Detection method of molecular weight of synthetic antigen, and application of method

The invention discloses a simple detection method of the molecular weight of a synthetic antigen. A standard curve is drafted through gel chromatography, and the standard curve is used to detect the molecular weight of antigen molecules. The detection method of the molecular weight of the synthetic antigen can be applied to detect the synthetic antigen, antibodies and protein macromolecular substances, and the detected molecular weight range is 16-150 kDa. Coupling proportions of micro-molecules and proteins in the synthesis process can be reckoned by detecting the molecular weight of the synthetic antigen and combining the molecular weights of the coupled proteins and micro-molecules. Compared with present detection methods, the method disclosed in the invention has the advantages of simplicity in operation, extremely small data processing difficulty, and reliable detection result. The method can be used to separate substance components with different molecular weights and calculate the molecular weights, an