Probe for human EGFR genetic mutation detection, kit and detection method thereof
The invention relates to the field of gene detection, in particular to a probe for human EGFR genetic mutation detection, a kit and a detection method thereof. The probe for human EGFR genetic mutation detection provided by the invention comprises any one or several kinds of probes in 1 to 35 probe...
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| creator | LIAO WEI LYU JIAHAN ZHANG CHENGUANG MO YAQIN |
| description | The invention relates to the field of gene detection, in particular to a probe for human EGFR genetic mutation detection, a kit and a detection method thereof. The probe for human EGFR genetic mutation detection provided by the invention comprises any one or several kinds of probes in 1 to 35 probe pairs. Each probe pair comprises a capture probe and a detection probe; the nucleotide sequences of the 1 to 35 probe pairs are shown as SEQ ID NO: 1 to 70. The probes all use hotspot mutation design on the human EGFR, and are obtained through multi-time screening; the capture probe and the detection probe are used in a matched way; the advantages of high sensitivity, high specificity and accurate and reliable results are realized. The invention also provides a kit for human EGFR genetic mutation detection. The kit only needs few detection samples; in the detection process, the electric field effect is utilized, the reaction speed is accelerated; the reaction time is shortened; the whole detection process can be co |
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The probe for human EGFR genetic mutation detection provided by the invention comprises any one or several kinds of probes in 1 to 35 probe pairs. Each probe pair comprises a capture probe and a detection probe; the nucleotide sequences of the 1 to 35 probe pairs are shown as SEQ ID NO: 1 to 70. The probes all use hotspot mutation design on the human EGFR, and are obtained through multi-time screening; the capture probe and the detection probe are used in a matched way; the advantages of high sensitivity, high specificity and accurate and reliable results are realized. The invention also provides a kit for human EGFR genetic mutation detection. The kit only needs few detection samples; in the detection process, the electric field effect is utilized, the reaction speed is accelerated; the reaction time is shortened; the whole detection process can be co</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; VINEGAR ; WINE</subject><creationdate>2017</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170215&DB=EPODOC&CC=CN&NR=106399518A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25564,76547</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170215&DB=EPODOC&CC=CN&NR=106399518A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LIAO WEI</creatorcontrib><creatorcontrib>LYU JIAHAN</creatorcontrib><creatorcontrib>ZHANG CHENGUANG</creatorcontrib><creatorcontrib>MO YAQIN</creatorcontrib><title>Probe for human EGFR genetic mutation detection, kit and detection method thereof</title><description>The invention relates to the field of gene detection, in particular to a probe for human EGFR genetic mutation detection, a kit and a detection method thereof. 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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE |
| title | Probe for human EGFR genetic mutation detection, kit and detection method thereof |
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