Probe for human EGFR genetic mutation detection, kit and detection method thereof

The invention relates to the field of gene detection, in particular to a probe for human EGFR genetic mutation detection, a kit and a detection method thereof. The probe for human EGFR genetic mutation detection provided by the invention comprises any one or several kinds of probes in 1 to 35 probe pairs. Each probe pair comprises a capture probe and a detection probe; the nucleotide sequences of the 1 to 35 probe pairs are shown as SEQ ID NO: 1 to 70. The probes all use hotspot mutation design on the human EGFR, and are obtained through multi-time screening; the capture probe and the detection probe are used in a matched way; the advantages of high sensitivity, high specificity and accurate and reliable results are realized. The invention also provides a kit for human EGFR genetic mutation detection. The kit only needs few detection samples; in the detection process, the electric field effect is utilized, the reaction speed is accelerated; the reaction time is shortened; the whole detection process can be co