Germacrone alcoholization and biological resolution method

The invention discloses a germacrone alcoholization and biological resolution method. The method comprises the following steps: by taking germacrone extracted from cedar branches and leaves as a raw material, carrying out reduction with sodium borohydride to obtain a compound II, carrying out enzymatic kinetic resolution reaction on the compound II to obtain a compound III and a compound IV, or carrying out dynamic kinetic resolution to obtain a compound III of which the yield is more than 90%, and hydrolyzing the compound III to obtain a compound V. According to the invention, a latent chiral ketone group in germacrone is further changed to a chiral hydroxyl center so as to carry out further resolution. The method disclosed by the invention has the characteristics of simple operation, high product yield, good optical purity and the like. 本发明公开了种杜鹃酮醇化以及生物拆分方法。本发明是以香柏枝叶中提取的杜鹃酮为原料，经硼氢化钠还原得化合物Ⅱ，化合物Ⅱ再通过酶催化的动力学拆分反应得化合物Ⅲ以及化合物Ⅳ，或经动态动力学拆分得收率90%以上的化合物Ⅲ，化合物Ⅲ经水解可得化合物Ⅴ。本发明近步将杜鹃酮中的潜手性酮基变成手性羟基中心，并进步进行拆分；本发明具备操作简单，产品收率高、光学纯