Porcine-intestinal microorganism specific interactive gene 1-38 for indicating porcine fecal pollution and application thereof

The invention relates to the field of porcine fecal pollutant detection, and particularly relates to a porcine-intestinal microorganism specific interactive gene 1-38 for indicating porcine fecal pollution and application thereof. The sequence of the porcine-intestinal microorganism specific interac...

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Hauptverfasser: MO HONGFEI, SHUAI JIANGBING, HE YONGQIANG, ZENG RUOXUE, ZHANG XIAOFENG
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention relates to the field of porcine fecal pollutant detection, and particularly relates to a porcine-intestinal microorganism specific interactive gene 1-38 for indicating porcine fecal pollution and application thereof. The sequence of the porcine-intestinal microorganism specific interactive gene 1-38 for indicating porcine fecal pollution is as shown in SEQ ID NO:1. According to the invention, porcine specific metagenomes are enriched through a competitive hybridization genome fragment enrichment (GFE) method to construct a metagenome library, and sequence flora and function classification is performed on the library, thereby realizing targeted screening of the host (porcine)-intestinal microorganism interactive target spot related specific gene, designing a molecular marker for the porcine specific interactive gene, and establishing a monitoring method capable of efficiently indicating a fecal pollution source with high sensitivity and strong specificity. 本发明涉及猪粪便污染物检测领域,尤其涉及种指示猪粪便污染的猪-肠道微生物特异性互