Mouse prostate cancer circulating tumor cell line and prostate cancer circulating tumor cell isolating and culturing method

The invention discloses a mouse prostate cancer circulating tumor cell line and a prostate cancer circulating tumor cell isolating and culturing method. The preservation number of the circulating tumor cell line is CCTCC C201601. The isolating and culturing method comprises the following steps: establishing an in-situ prostate cancer animal model, taking blood, lysing red blood cells, and conducting isolating and culturing and passage. Obtained circulating tumor cells are uniform in quality; based upon immunofluorescence as well as scratch and membrane-penetrating experiments, the obtained circulating tumor cells conform to the properties of the circulating tumor cells and the circulating tumor cells are relatively strong in transfer and invasion capacities; and the circulating tumor cells can serve as a good experimental tool for researching a prostate cancer transfer mechanism in a laboratory and can also provide a cell research model for preventing prostate cancer transfer in the clinical field. The circul