Polyphyllin-VI-producing Escherichia coli clx-6 strain and application thereof

The invention provides a polyphyllin-VI-producing Escherichia coli clx-6 strain, belonging to Escherichia sp.. The strain is collected by China Center for Type Culture Collection; the address is Wuhan University, Wuhan City, PRC; the postal code is 430072; the collection date is September 21st, 2016; the collection number is CCTCC NO:M2016508; and the 16S rDNA (ribosomal deoxyribonucleic acid) sequence of the strain is disclosed as SEQ ID NO.1. The Escherichia coli clx-6 strain can be used for preparing polyphyllin VI by fermentation. The culture medium for microbial fermentation is composed of 12 g/L sucrose, 15 g/L bean pulp and 15 g/L MgCl2. The optimal culture conditions are as follows: the pH value is 6.0, the culture temperature is 34 DEG C, and the shaking table rotation speed is 150 r/min. 本发明提供了种产重楼皂苷Ⅵ的大肠杆菌CLX-6菌株，属种大肠杆菌(Escherichia sp.)，该菌株保藏于中国典型培养物保藏中心，地址：中国，武汉，武汉大学；邮编430072，保藏日期为2016年9月21日，保藏编号为：CCTCC NO：M2016508；该菌株的16S rDNA序列如SEQ ID NO.1所示。上述大肠杆菌clx-6菌株能够用于发酵制备重楼皂苷Ⅵ。微生物发酵所采用的培养基配方为：蔗糖12g/L，豆粕15