Porcine epidemic diarrhea virus monoclonal antibody, cell strain thereof and application

The invention discloses a porcine epidemic diarrhea virus monoclonal antibody, a cell strain thereof and an application. The cell strain PEDV-J11E is collected at the China Center for Type Culture Collection, the collection number of the cell strain is CCTCC NO: C2016145, the monoclonal antibody secreted by the cell strain has high specificity and affinity for PEDV, can be specifically combined with different epitope of the PEDV and can be used for building a double-antibody sandwich ELISA (enzyme-linked immuno sorbent assay) method for quantitative detection of the PEDV. The double-antibody sandwich ELISA method has excellent detection specificity, sensitivity and linear detection range, and a double-antibody sandwich ELISA kit has excellent popularization and application values. 本发明公开了抗猪流行性腹泻病毒的单克隆抗体及其细胞株和应用。所述细胞株PEDV-J11E保藏于中国典型培养物保藏中心，保藏号为CCTCC NO:C2016145。其分泌的单克隆抗体对PEDV具有高特异性和高亲和力，能特异性结合PEDV的不同抗原表位，可用于建立双抗体夹心ELISA检测方法对PEDV进行定量检测。本发明建立的双抗体夹心ELISA方法具有很好的检测特异性和灵敏度，且具有很好的线性检测范围，说明本发明双抗体夹心ELISA试剂盒具有很好的推广应用价值。