Tuberculosis T cell detection kit and detection method thereof

The invention discloses a tuberculosis T cell detection kit. The kit uses a mouse-anti-human CD3 monoclonal antibody solution prepared by dissolving through a serum-free medium as positive quality control, and the concentration of the mouse-anti-human CD3 monoclonal antibody solution is 20-50 mu g/mL. Meanwhile, the invention discloses a detection method of the kit. A mouse-anti-human CD3 monoclonal antibody produced by hybridomas of which sources are stable and reliable is used as a positive control substance in the tuberculosis infection T cell diagnosis kit (enzyme linked immunospot assay), production conditions are controllable, spots are produced by nonspecific stimulation stably and effectively, costs of the tubercle bacillus detection kit are greatly reduced, and sources of the positive control substance are convenient and reliable. 本发明公开了种结核T细胞检测试剂盒，该试剂盒以无血清培养基溶解配制的鼠抗人CD3单克隆抗体溶液为阳性质控，所述鼠抗人CD3单克隆抗体溶液的浓度为20～50μg/mL，同时公开了该试剂盒的检测方法。本发明采用来源稳定可靠的杂交瘤细胞生产的鼠抗人CD3单克隆抗体作为结核感染T细胞诊断试剂盒(酶联免疫斑点法)中的阳性控制物，生产条件可控，非特异刺激