Soluble porcine epidemic diarrhea virus N protein and application thereof
The invention relates to a soluble porcine epidemic diarrhea virus N protein and an application thereof. A nucleotide sequence of the protein is shown as a SEQ ID No.1, a protein sequence is shown as a SEQ ID No.2, the protein is used for detecting a porcine epidemic diarrhea virus antibody, and the...
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| creator | LI LIMIN ZHU WEIXIA LI TANQING YUAN WANZHE SONG QINYE |
| description | The invention relates to a soluble porcine epidemic diarrhea virus N protein and an application thereof. A nucleotide sequence of the protein is shown as a SEQ ID No.1, a protein sequence is shown as a SEQ ID No.2, the protein is used for detecting a porcine epidemic diarrhea virus antibody, and the detection employs an indirect ELISA method. The porcine epidemic diarrhea virus N protein has a soluble form, is in favor of purifying protein, and can better guarantee biological activity of the protein. The provided detection method has stable detection result and good repeatability, and has further exploitation and application values.
本发明涉及种可溶性猪流行性腹泻病毒N蛋白及其应用，该蛋白核苷酸序列如SEQ ID No.1，蛋白序列如SEQ ID No.2，其用于猪流行性腹泻病毒抗体检测，具体采用间接ELISA的方法。本发明的猪流行性腹泻病毒N蛋白为可溶性形式，有利于蛋白的纯化，而且能够更好地保证蛋白的生物学活性；本发明提供的检测方法，检测结果稳定，具有良好的重复性，有进步开发应用价值。 |
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本发明涉及种可溶性猪流行性腹泻病毒N蛋白及其应用，该蛋白核苷酸序列如SEQ ID No.1，蛋白序列如SEQ ID No.2，其用于猪流行性腹泻病毒抗体检测，具体采用间接ELISA的方法。本发明的猪流行性腹泻病毒N蛋白为可溶性形式，有利于蛋白的纯化，而且能够更好地保证蛋白的生物学活性；本发明提供的检测方法，检测结果稳定，具有良好的重复性，有进步开发应用价值。</description><language>chi ; eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS THEREOF ; CULTURE MEDIA ; ENZYMOLOGY ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PHYSICS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>2017</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170118&DB=EPODOC&CC=CN&NR=106336451A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,780,885,25563,76418</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=20170118&DB=EPODOC&CC=CN&NR=106336451A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>LI LIMIN</creatorcontrib><creatorcontrib>ZHU WEIXIA</creatorcontrib><creatorcontrib>LI TANQING</creatorcontrib><creatorcontrib>YUAN WANZHE</creatorcontrib><creatorcontrib>SONG QINYE</creatorcontrib><title>Soluble porcine epidemic diarrhea virus N protein and application thereof</title><description>The invention relates to a soluble porcine epidemic diarrhea virus N protein and an application thereof. A nucleotide sequence of the protein is shown as a SEQ ID No.1, a protein sequence is shown as a SEQ ID No.2, the protein is used for detecting a porcine epidemic diarrhea virus antibody, and the detection employs an indirect ELISA method. The porcine epidemic diarrhea virus N protein has a soluble form, is in favor of purifying protein, and can better guarantee biological activity of the protein. The provided detection method has stable detection result and good repeatability, and has further exploitation and application values.
本发明涉及种可溶性猪流行性腹泻病毒N蛋白及其应用，该蛋白核苷酸序列如SEQ ID No.1，蛋白序列如SEQ ID No.2，其用于猪流行性腹泻病毒抗体检测，具体采用间接ELISA的方法。本发明的猪流行性腹泻病毒N蛋白为可溶性形式，有利于蛋白的纯化，而且能够更好地保证蛋白的生物学活性；本发明提供的检测方法，检测结果稳定，具有良好的重复性，有进步开发应用价值。</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS THEREOF</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PHYSICS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>2017</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNqNyrEKwjAQBuAsDqK-w_kAgiXavRRFly66lzP5Sw9iciSpz-_iAzh9y7c290cKyyuANGUnEQQVj7c48sI5z2D6SF4KDaQ5VUgkjp5YNYjjKilSnZGRpq1ZTRwKdj83Zn-9PPvbAZpGFGWHiDr2Q3NsrW1P56az_5wv8s00zA</recordid><startdate>20170118</startdate><enddate>20170118</enddate><creator>LI LIMIN</creator><creator>ZHU WEIXIA</creator><creator>LI TANQING</creator><creator>YUAN WANZHE</creator><creator>SONG QINYE</creator><scope>EVB</scope></search><sort><creationdate>20170118</creationdate><title>Soluble porcine epidemic diarrhea virus N protein and application thereof</title><author>LI LIMIN ; ZHU WEIXIA ; LI TANQING ; YUAN WANZHE ; SONG QINYE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_CN106336451A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>chi ; eng</language><creationdate>2017</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS THEREOF</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PHYSICS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>LI LIMIN</creatorcontrib><creatorcontrib>ZHU WEIXIA</creatorcontrib><creatorcontrib>LI TANQING</creatorcontrib><creatorcontrib>YUAN WANZHE</creatorcontrib><creatorcontrib>SONG QINYE</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>LI LIMIN</au><au>ZHU WEIXIA</au><au>LI TANQING</au><au>YUAN WANZHE</au><au>SONG QINYE</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Soluble porcine epidemic diarrhea virus N protein and application thereof</title><date>2017-01-18</date><risdate>2017</risdate><abstract>The invention relates to a soluble porcine epidemic diarrhea virus N protein and an application thereof. A nucleotide sequence of the protein is shown as a SEQ ID No.1, a protein sequence is shown as a SEQ ID No.2, the protein is used for detecting a porcine epidemic diarrhea virus antibody, and the detection employs an indirect ELISA method. The porcine epidemic diarrhea virus N protein has a soluble form, is in favor of purifying protein, and can better guarantee biological activity of the protein. The provided detection method has stable detection result and good repeatability, and has further exploitation and application values.
本发明涉及种可溶性猪流行性腹泻病毒N蛋白及其应用，该蛋白核苷酸序列如SEQ ID No.1，蛋白序列如SEQ ID No.2，其用于猪流行性腹泻病毒抗体检测，具体采用间接ELISA的方法。本发明的猪流行性腹泻病毒N蛋白为可溶性形式，有利于蛋白的纯化，而且能够更好地保证蛋白的生物学活性；本发明提供的检测方法，检测结果稳定，具有良好的重复性，有进步开发应用价值。</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | Soluble porcine epidemic diarrhea virus N protein and application thereof |
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