Recombinant expression vector, host and construction method thereof, and animal model construction method

The invention relates to the field of biotechnology, in particular to a recombinant expression vector, a host and a construction method thereof, and an animal model construction method. The recombinant expression vector consists of nucleotide for encoding soluble humanized HLA-A2 proteins and a prokaryotic expression vector. By virtue of the special prokaryotic expression vector, 'sufficient' soluble humanized HLA-A2 proteins can be synthesized; by taking the purified HLA-A2 proteins as an immunogen, the 'accuracy' of an experiment is guaranteed; the HLA-A2 proteins at different concentrations can promote a recipient mouse to generate preformed HLA-A2 antibodies at different titers, so that the generating strength of the preformed antibodies becomes 'controllable'; and the change of 'lymphocytes in marrow and spleen' of a preformed antibody animal model can be successfully observed. 本发明涉及生物技术领域，特别涉及重组表达载体、宿主及其构建方法以及动物模型的构建方法。该重组表达载体由编码可溶性人源化HLA-A2蛋白的核苷酸和原核表达载体构成。特有的原核表达体系合成"足量"可溶性人源化HLA-A2蛋白；采用纯化后的HLA-A2蛋白作为免