Method for testing riboflavin in yolk and VB2 tablet through molecular fluorescence difference labeling

The invention discloses a method for testing riboflavin in yolk and VB2 tablets through molecular fluorescence difference labeling. By adopting the method, a working curve does not need to be drawn, the mass percentage of a component to be tested can be rapidly acquired by only using two differential labeled test liquids, sample background interference is completely eliminated in the testing process, and meanwhile, as different amounts of standard solutions are added into two test liquids of the same volume, testing errors introduced by side reaction such as polymerization, dissociation and photolysis caused by different concentrations of the tested component in a sample can be compensated or deducted, the testing accuracy can be further ensured and improved. The detection limit of the method disclosed by the invention is 5.76ng/mL, and the limit of quantitation is 1.92*10mu g/mL. 种利用分子荧光差异加标测定蛋黄、VB药片中核黄素的方法，无需绘制工作曲线，只需通过两个差异加标的试液即可快速获得待测组分的质量分数，测定过程中完全消除了样品背景干扰，同时，采用向两个同体积试液中加入不同量的标准溶液，样品中因被测组分浓度不同可能因发生聚