Horse sperm low-temperature and freeze preservation diluent

The invention belongs to the field of biotechnology and relates to preparation of animal sperm low-temperature and freeze preservation diluents, in particular to a horse sperm low-temperature and freeze preservation liquid. The sperm low-temperature and freeze preservation diluent is prepared by combining sodium bicarbonate, hydroxyethylpiperazine ethane sulfonic acid, trisodium citrate dihydrate, potassium citrate monohydrate, sodium hydroxide, lactose, glucose, inositol, gentamicin, neomycin, apramycin, colistin, amphotericin, sodium pyrophosphate, sodium caseinate, whey protein, an antioxidant and deionized water. The horse sperm low-temperature and freeze preservation diluent can effectively increase the survival rate of sperms obtained after unfreezing of low-temperature and freezing of horse seminal fluid and the hybridization conception rate. Compared with a conventional method, the method is easy and stable to implement and especially remarkable in technical effect. 本发明属于生物技术领域，涉及动物精液低温和冷冻保存液的制作，特别涉及种