Bladder membrane biological scaffold, preparation method and purpose thereof

The invention provides a bladder membrane biological scaffold preparation method. The method comprises the following steps: 1) using NaOH for processing bladder tissues; 2) using TritonX-100 and SDS for processing the bladder tissues with any sequence, repeating the step; 3) using NaOH for processin...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
1. Verfasser: WEI GUIQING
Format: Patent
Sprache:chi ; eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The invention provides a bladder membrane biological scaffold preparation method. The method comprises the following steps: 1) using NaOH for processing bladder tissues; 2) using TritonX-100 and SDS for processing the bladder tissues with any sequence, repeating the step; 3) using NaOH for processing the bladder tissues; and 4) drying the product at low temperature. In the method, a protease inhibitor can be used for processing the bladder tissues before the step 1), and nuclease can be used for processing the bladder tissues after processing by TritonX-100 and SDS in the step 2). The prepared bladder membrane biological scaffold has good biocompatibility and mechanical strength, and provides a tissues regenerating framework, and has the advantages that bacteria and virus can be thoroughly killed, the rejection is little, and the bladder membrane biological scaffold can be used for bladder restoration. 本发明提供种制备膀胱膜生物支架的方法,包括(1)用NaOH处理膀胱组织;(2)用TritonX-100和SDS以任意顺序处理所得物,并重复遍该步骤;(3)用NaOH处理所得物;(4)将所得物低温干燥。在该方法中,可以