Method for distinguishing and analyzing components in cockroaches
The invention provides a method for distinguishing and analyzing components in cockroaches. The method comprises the specific steps that 1, cockroach samples of male adults, female adults, just exuviated adults, nymphs of 7-8 days old, and nymphs of 3-4 days old are collected; 2, the cockroach sampl...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention provides a method for distinguishing and analyzing components in cockroaches. The method comprises the specific steps that 1, cockroach samples of male adults, female adults, just exuviated adults, nymphs of 7-8 days old, and nymphs of 3-4 days old are collected; 2, the cockroach samples in different stages are prepared: extraction is performed by using ethyl acetate, and then methyl esterification treatment is performed; 3, GC-MS analysis conditions are optimized: the GC-MS analysis conditions for analyzing the components in cockroaches in different stages are optimized by selecting sample inlet temperature, sample size and sampling modes; 4, the specific components of the cockroaches in each stage are distinguished. The method is high in sensitivity and good in component separating effect, distinguishing results are stable and reliable, the unique components and common components of the cockroaches in different stages are fully explored, and improvement of cockroach research and utilization is |
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