Poultry intestinal tract Bacillus coagulans quantitative detection kit and detection method

The invention discloses a poultry intestinal tract Bacillus coagulans quantitative detection kit and detection method, belonging to the technical field of microbial detection. By using the conserved sequence of the Bacillus coagulans genome 18S rDNA as the target detection segment, a bioinformatical process is utilized to design and synthesize the specific primer sequence for covering the target segment; and a fluorescent real-time quantitative nucleic acid amplification technique is combined to quantitatively analyze the Bacillus coagulans in the poultry intestinal tract. Compared with other intestinal microbe counting methods, the method disclosed by the invention is simple to operate, has the advantages of high speed, high efficiency, high specificity, high accuracy, high economy, high practicality and the like, and is suitable for high-flux detection and analysis. 本发明公开了种禽类肠道凝结芽孢杆菌定量检测试剂盒及检测方法，属于微生物检测技术领域。本发明以凝结芽孢杆菌基因组18S rDNA中保守序列为目标检测片段，采用生物信息学方法设计合成覆盖目标片段的特异性引物序列，结合荧光实时定量核酸扩增技术，定量分析禽类肠道内凝结芽孢杆菌。与其他肠道微生物