Melanoma antigenic specificity CTL and preparation method thereof

The invention discloses a melanoma antigenic specificity CTL and a preparation method thereof. The preparation method of the melanoma antigenic specificity CTL includes the steps that peripheral blood mononuclear cells are obtained; mature DC cells loading melanoma antigenic polypeptides are prepared; CD8+T lymphocytes are obtained from the peripheral blood mononuclear cells; the mature DC cells loading the melanoma antigenic polypeptides are used for stimulating the CD8+T lymphocytes; the stimulated CD8+T lymphocytes are marked through the Tetramer technology, and Tetramer+CD8+ lymphocytes are separated out; the Tetramer+CD8+ lymphocytes are stimulated to prepare the melanoma antigenic specificity CTL. The preparation method of the melanoma antigenic specificity CTL is short in preparation time and high in expansion multiple number, the purity of the CTL is as high as 98%, and the melanoma cell killing effect is good. 本发明公开了种黑色素瘤抗原特异性CTL及其制备方法，其中，所述黑色素瘤抗原特异性CTL的制备方法包括：获取外周血单核细胞；制备负载黑色素瘤抗原多肽的成熟DC细胞；从所述外周血单核细胞