Method for increasing protein crystallization success rate by applying cyclodextrin as seed crystals

The invention provides a method for increasing the protein crystallization success rate by applying cyclodextrin as seed crystals. The method comprises the steps that firstly, a protein solution is prepared; secondly, the cyclodextrin is added, and a protein crystallization system is established; finally, observation under a microscope is conducted, and the number of conditions of generating protein crystals is counted. According to the method, by adding the cyclodextrin and derivatives thereof into the crystallization system, the crystal nucleation probability is increased, and then the protein crystallization success rate is effectively increased; the highest crystallization success rate can be increased by 5 times or above of a control group without adding the cyclodextrin in the prior art. 本发明提供了种使用环糊精作为籽晶来提高蛋白质结晶成功率的方法，首先配置蛋白质溶液，然后添加环糊精，建立蛋白质结晶体系，最终在显微镜下观察，统计出现蛋白质晶体的条件数目。本发明通过将环糊精及其衍生物添加在结晶体系中，使得晶体形核几率增加，从而有效地提高了蛋白质结晶成功率。通过本发明，结晶成功率的提高最大可达到现有技术中未添加环糊精的对照组的5倍以上。