Freeze-drying preparation of plasminogen and preparation method of freeze-drying preparation of plasminogen

The invention provides a freeze-drying preparation of plasminogen, in particular to a preparation method of the freeze-drying preparation of plasminogen. The preparation method is characterized by including the steps: (1) taking purified plasminogen samples, using buffer solution containing sodium chloride, sodium citrate and trometamol with the pH being 6.0-7.5 for ultrafiltration dialysis, and concentrating till the centration of plasminogen is 3-6%; (2) adding glycine, mannitol and sucrose or combination thereof as well as sodium chloride, sodium citrate and trometamol into the solution obtained in the step (1) to commonly form a freeze-drying protective agent; (3) using a degerming filter membrane of 0.45 microns and 0.2 microns for filtering prior to freeze drying. 本发明提供了种人纤维蛋白溶解酶原的冻干制剂，具体地种人纤维蛋白溶解酶原的冻干制剂的制备方法，其特征在于，步骤为：1)取纯化后的人纤维蛋白溶解酶原样品，用含有氯化钠、柠檬酸钠、氨基丁三醇，pH6.0～7.5的缓冲液进行超滤透析，浓缩至人纤维蛋白溶解酶原的浓度为3％～6％；2)在步骤1)所得溶液中添加甘氨酸、甘露醇、蔗糖或几种的组合与氯化钠、柠檬酸钠、氨基丁三醇共同构成冻干保护剂；3)用0.45μm和0.2μm除菌滤膜过滤后，进行冻干。