Sequencing method applicable to small sequencers

The invention discloses a sequencing method applicable to small sequencers. The sequencing method comprises steps as follows: a sample is sequenced with a microarray sequencing chip, at least two libraries are adopted during sample loading, the at least two libraries include a sequencing library and an occupation library, and the occupation library can be connected with the sequencing chip and has no area intersected with a sequencing primer; the sequencing library can be connected with the sequencing chip, is connected with a to-be-tested sample sequence and has an area intersected with the sequencing primer. According to the sequencing method, an evenly mixed solution consisting of the occupation library and sequencing library is adopted during sample loading of the libraries, the distance between the sequencing libraries is changed on the premise that the sequencing stability and uniformity are guaranteed, so that the sequencing method is applicable to different optical systems, particularly small sequence