Method for preparing tyrosine-O-sulfonate
The invention relates to a method for preparing tyrosine-O-sulfonate. The method comprises the following steps that 1, a sulfoating reagent reacts with Fmoc-protected tyrosine in the presence of a thinner and alkali to obtain a reaction solution; 2, the reaction solution obtained in the step 1 is po...
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Format: | Patent |
Sprache: | chi ; eng |
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Zusammenfassung: | The invention relates to a method for preparing tyrosine-O-sulfonate. The method comprises the following steps that 1, a sulfoating reagent reacts with Fmoc-protected tyrosine in the presence of a thinner and alkali to obtain a reaction solution; 2, the reaction solution obtained in the step 1 is poured into a poor solvent, and then standing is conducted; 3, a supernatant solution of a product obtained in the step 2 is poured out, residues are dissolved with water, and the pH is regulated to be weakly acidic through alkali; 4, an aqueous solution obtained in the step 3 is washed with a good solvent and then concentrated; 5, the good solvent is added into a concentrate product obtained in the step 4, stirring and filtering are sequentially conducted, filtrate is added into the poor solvent, stirring is conducted, solids are collected and dried, and the solid product is obtained. According to the method, the problems that when distillation, freeze-drying and the like are applied, long time is consumed, and the |
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