Method suitable for sterilizing and inoculating trace orchid seeds in in-vitro preservation process

The invention discloses a method suitable for sterilizing and inoculating trace orchid seeds in the in-vitro preservation process. Through the combination of the sterilizing method of the tissue culture technology and DNA purification column and other tools, the method particularly suitable for ster...

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Bibliographische Detailangaben
Hauptverfasser: YANG DUNBEI, LI CUNFU, HE DUN, MENG JING, LI DEZHU
Format: Patent
Sprache:chi ; eng
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Zusammenfassung:The invention discloses a method suitable for sterilizing and inoculating trace orchid seeds in the in-vitro preservation process. Through the combination of the sterilizing method of the tissue culture technology and DNA purification column and other tools, the method particularly suitable for sterilizing and inoculating pod-cracking fine trace orchid seeds is designed. According to the technology, the problems of insufficient sterilization, seed mixing and disinfectant residual easily occurring in the disinfection process of orchid seeds are solved, disinfecting and inoculating of trace orchid seeds can be efficiently conducted, the pollution rate is effectively decreased, and the method has the advantages that experiment equipment is easy to obtain, cost is low, operation is convenient, and inoculation efficiency is high. 本发明公开了种兰科植物微量种子的灭菌及接种方法。结合组织培养技术的灭菌方法和DNA纯化柱等工具,设计了套特别适用于果荚开裂、细小且微量的兰科植物种子的灭菌和接种方法。该技术克服了兰科植物种子在消毒过程中易出现的灭菌不充分、混种及消毒液残留的问题,能高效地进行微量的兰科植物种子的消毒和接种,有效降低污染率,具有实验器材易得、成本低廉、操作方便、接种效率高等优点。